Coding
Str(e)pF

Part:BBa_K4590008:Design

Designed by: Beatriz Lauriano de Oliveira   Group: iGEM23_USP-Brazil   (2023-10-02)


di-Tricarboxylate Transporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1072
    Illegal NotI site found at 1213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 461
    Illegal BamHI site found at 989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 439
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 291
    Illegal SapI site found at 525
    Illegal SapI site found at 774


Design Notes

We chose the gene from another Streptomyces with the ability to incorporate malonate.


Source

Streptomyces hygroscopicus var. ascomyceticus FK520 biosynthetic gene cluster, partial sequence GenBank: AF235504.1

References